Renin, also known as angiotensinogenase, is an enzyme that the kidneys produce to regulate aldosterone production. It’s also essential in the renin–angiotensin aldosterone system (RAAS) which maintains your body’s fluid balance and blood pressure. Aldosterone is the primary of several endogenous members of the class of mineralocorticoids in humans. Aldosterone tends to promote sodium and water retention, and lower plasma postasium concentrations.

Insulin-like Growth Factor 1, also known as IGF-1, is used as a biomarker for blood levels of human growth hormone. It is a hormone produced by the liver and other tissues which affects growth-promoting activities and glucose metabolism. The blood level of IGF-1 appears to fluctuate over 24 hours and is found to be related to growth hormone secretion.

The adrenal gland produces a hormone known as dehydroepiandrosterone (DHEA). It is also made in the brain. DHEA leads to the production of androgens and estrogens (male and female sex hormones). DHEA levels in the body begin to decrease after age 30. Lower DHEA levels are found in people with hormonal disorders, HIV/AIDS, Alzheimer's disease, heart disease, depression, diabetes, inflammation, immune disorders, and osteoporosis. Corticosteroids, birth control taken by mouth, and agents that treat psychiatric disorders may reduce DHEA levels.

This test measures the amount of dihydrotestosterone (DHT) in the blood.  Low DHT has been associated with low libido and high DHT has been linked to acne, hair loss and benign prostatic inflammation in a minority of men.