This hormone panel for men on testosterone replacement therapy (TRT) contains upstream and downstream hormones to testosterone.  TRT has been shown to decrease upstream hormones like pregnenolone and progesterone, and slightly decrease DHEA. Downstream hormone metabolites like ultrasensitive estradiol and DHT are also included.  Lastly, prolactin is added since high prolactin can decrease sexual function.  No LH and FSH were included since TRT shuts them down.

This panel includes: Testosterone, Total, LC/MS and Free (Equilibrium Dialysis), and 17-OH-progesterone, a metabolite that has been correlated with sperm production.

Testosterone Replacement Therapy (TRT) shuts down upstream hormones like LH and FSH required for sperm production, so men on TRT generally have a difficult time fathering a child. Some studies show that adding hCG to a TRT protocol may improve sperm production since hCG is an analog of LH that could stimulate sperm production. But optimizing hCG dose and frequency based on sperm count can be a time consuming process since it takes at least 8 weeks to see changes in sperm tests. Fortunately, there is an easier way by measuring 17-OH-progesterone to predict the efficacy of the hCG+TRT protocol without having to wait for 8 weeks between hCG dose/frequency changes.