Hormone Panel for Men on TRT- Comprehensive

Hormone Panel for Men on TRT- Comprehensive

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This hormone panel for men on testosterone replacement therapy (TRT) contains upstream and downstream hormones to testosterone.  TRT has been shown to decrease upstream hormones like pregnenolone and progesterone, and slightly decrease DHEA. Downstream hormone metabolites like ultrasensitive estradiol and DHT are also included.  Lastly, prolactin is added since high prolactin can decrease secual function.  No LH and FSH were included since TRT shuts them down.

This hormone panel for men on testosterone replacement therapy (TRT) contains upstream and downstream hormones to testosterone.  TRT has been shown to decrease upstream hormones like pregnenolone and progesterone, and slightly decrease DHEA. Downstream hormone metabolites like ultrasensitive estradiol and DHT are also included.  Lastly, prolactin is added since high prolactin can decrease sexual function.  No LH and FSH were included since TRT shuts them down.

You can add IGF-1 and 17-OH-progesterone tests to your cart for an even more complete picture.

  • DHEA-S Test

Dehydroepiandrosterone (DHEA) is a hormone produced by the adrenal gland. It is also made in the brain. DHEA leads to the production of androgens and estrogens (male and female sex hormones). DHEA levels in the body begin to decrease after age 30. Lower DHEA levels are found in people with hormonal disorders, HIV/AIDS, Alzheimer's disease, heart disease, depression, diabetes, inflammation, immune disorders, and osteoporosis. Corticosteroids, birth control taken by mouth, and agents that treat psychiatric disorders may reduce DHEA levels.  Do you need to take a DHEA supplement?


Estradiol is the predominant sex hormone present in females and is also found at lower levels in men. It represents the major estrogen in humans. Estradiol not only impacts reproductive and sexual functioning, but also affects other systems including bone health, heart health, nerves and metabolism. Estradiol, also known as E2, is the most active of all three estrogens commonly measured in a clinical setting. For women, it is important to compare the relationship between estradiol and progesterone in evaluating menopausal symptoms such as hot flashes, mood disorders, and aging skin. In both men and women, low levels of estradiol are associated with osteoporosis. More information: Estradiol in Men: Top Ten Things You Should Know


  • Progesterone Test

Progesterone is a female hormone important for the regulation of ovulation and menstruation. It is also present in men in smaller concentrations. This test is performed using the immunoassay technology. Progesterone is used to cause menstrual periods in women who have not yet reached menopause but are not having periods due to a lack of progesterone in the body. This hormone is also used to prevent overgrowth in the lining of the uterus in postmenopausal women who are receiving estrogen hormone replacement therapy. Progesterone replacement may also be used for other purposes. TRT may lower progesterone in men.  Progesterone may have a calming effect in men, so some researchers speculate that decreasing progesterone levels in men may affect mood, sleep quality and anxiety.


  • Pregnenolone Test

Pregnenolone is the mother of all hormones. It is known as a neurosteroid with brain health benefits.  As most hormones, it decreases with age. Aside from the natural decrease in pregnenolone that occurs as you age, research shows that higher doses of Testosterone Replacement Therapy (TRT) cause pregnenolone levels to drop even further. 

This pregnenolone lab test uses liquid chromatography, so it is accurate for the measurement of pregnenolone. Pregnenolone is known as the mother hormone. All other steroids including testosterone and estrogen are derived from this important hormone. For this reason, optimal blood levels of pregnenolone are critical for a healthy hormone balance.

However, pregnenolone does more. It is important for proper brain development, cognition, memory, and mood. These dramatic effects on the brain are why pregnenolone is known as a neuro-active steroid.

Fasting is not required. Pregnenolone supplementation should be taken approximately 2 hours before the blood draw. More on the role of pregenolone in men.


  • Total Testosterone, LC/MS

  • Free Testosterone by Equilibrium Ultrafiltration

This panel includes total testosterone measured by the most accurate method for women (liquid chromatography/mass spectrometry), free testosterone percent by equilibrium ultrafiltration, and free testosterone calculated from percent free T. Food may temporily low testosterone blood levels in men not on TRT.


  • Dihydrotestosterone (DHT) 

DHT is synthesized from testosterone in the prostate gland, testes, hair follicles, and adrenal glands by 5-alpha reductase. Dihydrotestosterone exerts its action similar to testosterone, which binds to and activates androgen receptors. Dihydrotestosterone is responsible for the formation of male primary sex characteristics and most male secondary sex characteristics during puberty, such as muscular growth, facial and body hair growth, and deepening of the voice.

Excess DHT has been associated with hair loss, prostate volume increase, and acne in men with a genetic predisposition to those issues. Blocking or decreasing DHT to low levels may cause sexual dysfunction. Please read DHT: Friend or Enemy of Men?



  • Prolactin Test

Prolactin is secreted by the pituitary gland to induce milk production. It also has many other functions on immune response, myelin coating on nerves, heart muscle protection, and enhances LH receptors on testicular Leydig cells so that testosterone can be produced. 

Prolactin levels peak during REM sleep, and in the early morning. Levels can rise after exercise, meals, sexual intercourse, minor surgical procedures, or following epileptic seizures. 

High prolactin can decrease testosterone and cause sexual dysfunction. Higher levels of prolactin have been associated with tumors involving the lungs, the pituitary gland, and the kidneys. Prolactin is sometimes used by doctors to evaluate sexual dysfunction of unknown cause in both men and women.

Drugs, supplements and certain diseases like pituitary adenomas may disrupt normal prolactin blood levels. These include hypothyroidism, antiepileptics, antidepressants, estrogen, opiates, diuretics, certain growth hormone releasing peptides and arginine. More on the role of prolactin in men.


  • TSH Test

The thyroid stimulating hormone (TSH) is produced and secreted by the pituitary gland and stimulates the thyroid to produce T3 and T4. When TSH is elevated, low thyroid function (hypothyroidism) may be indicated. If TSH is low it is indicative of high thyroid function (hyperthyroidism). However, using TSH by itself to diagnose thyroid function issues may not provide the whole picture. That is why free T3 and free T4 are important.

  • Free T3 Test

T3 or triiodothyronine is the active form of the thyroid hormone. T3 acts as a metabolic throttle for fueling all of the daily cellular functions. Free T3 is the unbound portion of T3 that is biologically active.

  • Free T4 Test

The thyroid gland produces and secretes T4, otherwise known as thyroxine. Proteins bind to T4 and carry it throughout the bloodstream. Once in the tissues, T4 is released from the proteins and is now free to convert into the more active form called T3. For this reason, many doctors believe that measuring free T4 is a more sensitive test for thyroid hormone production.

More on the use of thyroid function tests .



  1. Go to the "Find a Location" page to find the closest lab location. No need to make an appointment since walk-ins are welcomed. Once you have identified your closest location, go to step 2.

    NOTE: We serve all states in the U.S. except New York, New Jersey, and Rhode Island. Residents of those states need a written lab order from an in-state provider. Individuals cannot obtain a blood draw in neighboring states.
  2. Go to "Choose a Test" and add your selection (s) in the shopping cart. If you prefer to save money on bundled tests, we have created "Lab Test Panels" that can help you decide what to order.

  3. If you have a discount coupon code, add it to your cart.

  4. A $8 lab processing fee will be added to your total.

  5. Pay using a credit card.

  6. You will receive an order confirmation and instructions email on how to download your lab request.

  7. Print lab request form that you downloaded.

  8. Take that form to the closest Quest Diagnostics location with a picture ID. Get your blood drawn.

  9. You will receive an email when the results are ready for you to download.

    Note: You cannot place an order under someone else's profile. The profile person's name will appear on the lab order form.

    If you have questions, please review our FAQ Page.

    If you have further questions, please email [email protected].

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