Autoimmune Antibodies: ANA Screen, IFA, with Reflex to Titer and Pattern

Autoimmune Antibodies: ANA Screen, IFA, with Reflex to Titer

First time customer? Read How Discounted Labs Works 

IMPORTANT NOTICE ABOUT DISCOUNTED LABS SERVICES: We currently serve the following states:

Alabama, Arkansas, California, Colorado, Connecticut, Florida, Georgia, Illinois, Indiana, Iowa, Kansas, Kentucky, Louisiana, Maine, Maryland, Massachusetts, Michigan, Minnesota, Mississippi, Missouri, Montana, Nebraska, Nevada, North Carolina, New Mexico, Ohio, Oklahoma, Oregon, Pennsylvania, South Carolina, Tennessee, Texas, Utah, Virginia, Washington, and West Virginia.

Other states will be added in the coming months, so please subscribe to our newsletter on the website footer to be notified.

This test measures ANA Screen, IFA, with Reflex to Titer and Pattern - Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogren's syndrome, scleroderma, polymyositis, CREST syndrome, and neurologic SLE.

ANA Screen: Autoimmune Antibodies

This test measures ANA Screen, IFA, with Reflex to Titer and Pattern - Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogren's syndrome, scleroderma, polymyositis, CREST syndrome, and neurologic SLE.

An ANA test looks for antinuclear antibodies in your blood. If the test finds antinuclear antibodies in your blood, it may mean you have an autoimmune disorder. An autoimmune disorder causes your immune system to attack your own cells, tissues, and/or organs by mistake.

Antinuclear antibodies (ANA) are a group of autoantibodies produced by a person's immune system when it fails to adequately distinguish between "self" and "nonself." The ANA test detects these autoantibodies in the blood.

ANA react with components of the body's own healthy cells and cause signs and symptoms such as tissue and organ inflammation, joint and muscle pain, and fatigue. ANA specifically target substances found in the nucleus of a cell, hence the name "antinuclear." They probably do not damage living cells because they cannot access their nuclei. However, ANA can cause damage to tissue by reacting with nuclear substances when they are released from injured or dying cells.

The ANA test is one of the primary tests for helping to diagnose a suspected autoimmune disorder or rule out other conditions with similar signs and symptoms. The ANA test may be positive with several autoimmune disorders. Patients with the autoimmune disorder systemic lupus erythematosus (SLE) are almost always positive for ANA, but the percentage of patients with other autoimmune disorders who have positive ANA results varies. Also, a significant number of patients with a variety of other types of disorders (and even some healthy people) may be positive for ANA, especially at low levels.

The ANA test is ordered when someone shows signs and symptoms that a healthcare practitioner suspects are due to a systemic autoimmune disorder. People with autoimmune disorders can have a variety of symptoms that are vague and non-specific and that change over time, progressively worsen, or alternate between periods of flare ups and remissions.

Understanding the Basics of an ANA Screen IFA w/Refl Titer and Pattern Test


Although the term "ANA screen IFA w/refl titer and pattern" sounds like an overly complicated medical procedure, this is just a test to see if you have an autoimmune disease.

In plain language, autoimmune diseases happen when your body turns against you. This immune activation means that your immune system starts to attack your cells and tissues. Chronic autoimmune ailments can lead to serious health complications, so you should know more about them.

Keep reading to learn more about this type of test, the symptoms of autoimmune diseases, and available treatment options.

How Does an ANA Screen IFA w/Refl Titer and Pattern Test Work?

First of all, let's understand the basics of the ANA test. ANA stands for antinuclear antibodies, and IFA stands for Indirect Fluorescent Antibody. This type of test is designed to look for particles that can damage the nucleus of your own cells. In simpler words, the ANA test checks your body to see if you have cells sabotaging your system. Healthy people have some of these cells in the body, but if you have too many, problems start to appear.

But why would your body produce cells that are damaging your health in the first place? This is a good question. Scientists believe that abnormal genetic mutations happen in the body, leading to the production of cells that are harmful to your health. These cells damage the nucleus of other cells, particularly those already damaged and vulnerable. Your body detects this process and makes antinuclear antibodies.

The ANA test itself provides an excellent way to detect if you have an autoimmune disease. Autoimmune diseases damage healthy cells and tissues in your system. Some of the most popular ones are lupus, rheumatoid arthritis, celiac disease, and others. Although a positive result from an ANA test doesn't automatically confirm an autoimmune disease, it's the primary way of investigating these health issues. If you get a positive result, your doctor might order additional tests to detect what type of autoimmune disease you have and its severity.

How Are the Test Results Interpreted?

It's important to note that the reference ranges for the ANA test vary from laboratory to laboratory. For example, most experts agree that a positive result is considered a ratio of 1:60 or higher, but these numbers are heavily debated. It's important to discuss everything with your doctor and understand more about ANA test results. Even if your results are close to the limit, you should investigate the problem further and see if you're at risk for chronic and dangerous autoimmune disorders.

It's also worth noting that a positive result for an ANA test doesn't automatically mean that you're dealing with an autoimmune disease. More tests are required to see what organs and tissues are affected and the severity of your health problem. At the same time, healthy people might also get positive results from time to time. The ANA test is the launching pad for further health investigations, and that's why you should do it often, maybe once every year if you're able.

What Are the Most Common Autoimmune Diseases?

Medical doctors have identified and studied multiple autoimmune disorders, depending on what symptoms they cause and what tissues they attack. Here is a short list of some of the most widespread autoimmune diseases:

Celiac Disease

Celiac disease is an increasingly prevalent health problem caused by the body's inability to process gluten. Gluten is a type of protein added to foods to increase their consistency. However, too much can get stuck inside the stomach lining and cause inflammation. The body produces cells to solve this problem but attacks its tissues. Celiac disease is one of the most troublesome autoimmune disorders studied by medical experts.

Rheumatoid Arthritis

Rheumatoid arthritis is another autoimmune disease that can cause severe complications if left untreated. It causes various symptoms such as joint pain, chronic fatigue, reduced mobility, headaches, etc. Joint pain and swelling can manifest in hands, wrists, knees, and many other body parts.

Autoimmune Hepatitis

This type of disease affects one of the most essential organs in your body: your liver. Autoimmune hepatitis causes inflammation and swelling in your liver, reducing its ability to function correctly.

Addison Disease

Another common autoimmune illness is Addison's Disease. It affects the adrenal glands that are responsible for producing cortisol. Cortisol is also known as the stress hormone, and having too much of it can decrease the quality of life and make you feel constantly fatigued.

An ANA test can reveal if you're struggling with an autoimmune disorder affecting your adrenal glands. If you're feeling weak or tired most of the time, your adrenal glands might not function properly. Addison's disease is prevalent in the United States, but its symptoms can be easily managed with medication.

Systemic Lupus

You have probably already heard about lupus, a common autoimmune problem. It's a chronic disease that can affect various parts of your body, including your brain, lungs, blood vessels, kidneys, and others. Your immune system starts to see your organs and tissues as foreign invaders. This autoimmune disease triggers a series of inflammatory responses that causes pain, fatigue, swelling, and other symptoms.

What Are the Symptoms of Autoimmune Diseases?

Fortunately, most autoimmune problems also cause specific symptoms, so you know you're dealing with something serious. For example, some of the most common symptoms of autoimmune illnesses include low-grade fever and weakness. These symptoms can last for weeks or months, and you'll soon discover that a common cold or the flu does not cause it.

Autoimmune diseases can also cause joint pain and skin rashes. You might have an autoimmune disease if your wrists, knees, elbows, or other joints are aching. If you also experience muscle pain and tingling in your hands and feet, you should do an ANA test and see if an autoimmune issue is causing you these issues.

At the same time, autoimmune problems also cause many symptoms you cannot see immediately. These diseases increase inflammation in your body. As a result, chronic inflammation can affect your lungs, kidneys, liver, and many other organs and tissues. A simple inflammation test can reveal if specific inflammation biomarkers in your body might be elevated. If this is the case, you need to discover the root cause of your chronic inflammation, and in many cases, this can be attributed to an autoimmune disorder.

Finally, some people also experience hair loss as a result of dealing with an autoimmune problem. If you see large patches of hair on your pillow when you wake up, you should investigate this problem immediately. Doing an ANA test is an excellent way to explore your health problems.

Autoimmune Disorders Signs and Symptoms:

  • Low-grade fever
  • Persistent fatigue, weakness
  • Arthritis-like pain in one or more joints
  • Red rash (for lupus, one resembling a butterfly across the nose and cheeks)
  • Skin sensitivity to light
  • Hair loss
  • Muscle pain
  • Numbness or tingling in the hands or feet
  • Inflammation and damage to organs and tissues, including the kidneys, lungs, heart, lining of the heart, central nervous system, and blood vessels

How to Manage and Treat Autoimmune Conditions

Medical experts don't fully understand why many cells in the body start to attack healthy tissues. That's why sometimes treating or managing autoimmune disorders can be challenging. Most autoimmune diseases have limited treatment options, but you can manage their symptoms efficiently and live healthier lives.

For example, you need to find ways to bring the level of inflammation in the body to a lower level. Chronic inflammation is the source of most autoimmune disorders. You can reduce your inflammation levels by eating a healthier and balanced diet, avoiding sugary foods and drinks, and reducing alcohol intake. If you're a smoker, try to reduce the number of cigarettes you smoke every day, or even better, try quitting altogether.

Another way you can lower inflammation levels is by exercising more often and fast periodically. Talk to your doctor and see how intermittent fasting, for example, can reduce inflammation levels in the body. You can also manage the symptoms of autoimmune diseases by taking certain medications. However, ensure that you follow your doctor's advice, as certain drugs might cause additional damage to your health.

Order Your ANA Screen IFA Test Today!

As you can see, autoimmune diseases are not health issues that you can take lightly. When your body turns against you, it's necessary to do something right away to minimize damage as much as possible. The ANA Screen IFA w/Refl Titer and Pattern test provides an excellent opportunity to protect yourself against chronic autoimmune problems.

Go to and order your antinuclear antibodies test today. You'll find it affordable, and based on the test results, you will know exactly if you're at risk for autoimmune diseases.



Autoimmune Diseases: Signs, Symptoms, and Complications

Autoimmune Diseases: Types, Symptoms, Causes & More

Antinuclear Antibody (ANA) Test

ANA (Antinuclear Antibody) Test: MedlinePlus Medical Test






  1. Go to the "Find a Location" page to find the closest lab location. No need to make an appointment since walk-ins are welcomed. Once you have identified your closest location, go to step 2.

  2. Go to "Choose a Test" and add your selection (s) in the shopping cart. If you prefer to save money on bundled tests, we have created "Lab Test Panels" that can help you decide what to order.

  3. If you have a discount coupon code, add it to your cart.

  4. A $8 lab processing fee will be added to your total.

  5. Pay using a credit card.

  6. You will receive an order confirmation and instructions email on how to download your lab request.

  7. Print lab request form that you downloaded.

  8. Take that form to the closest location. Get your blood drawn.

  9. You will receive an email when the results are ready for you to download.

    Note: You cannot place an order under someone else's profile. The profile person's name will appear on the lab order form.


    If you have further questions, please email [email protected].