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SHBG (sex hormone binding globulin) binds to testosterone and estradiol. High SHBG may decrease free testosterone and free estradiol.

Pregnenolone is known as the mother hormone. All other steroids including testosterone and estrogen are derived from this important hormone. For this reason, optimal blood levels of pregnenolone are critical for a healthy hormone balance.  This test uses liquid chromatography/mass spectrometry, the most accurate assay for hormones.