Cortisol (hydrocortisone) is the most prominent glucocorticosteroid, and it is essential for the maintenance of several body functions. The most important physiological effects of cortisol are that it raises blood sugar levels (enhances gluconeogenesis, or breaks down glucose) and lowers inflammation and the immune system.

The serum iron test measures the amount of iron in your blood. The total iron-binding capacity (TIBC) test looks at how well the iron moves through your body.

Vitamin B-12 is an essential vitamin. Low blood levels of B12 can cause: Anemia and pernicious anemia, loss of balance , numbness or tingling in the arms and legs, and weakness

T3 or triiodothyronine is the active form of the thyroid hormone. T3 acts as a metabolic throttle for fueling all of the daily cellular functions. Free T3 is the unbound portion of T3 that is biologically active.

The thyroid stimulating hormone (TSH) is produced and secreted by the pituitary gland and stimulates the thyroid to produce T3 and T4. When TSH is elevated, low thyroid function may be indicated. If TSH is low it is indicative of high thyroid function. A study found that TSH was suppressed in all subjects after food, so fasting may be suggested although it is not reqired by Quest. Free T4 and Free T4 values did not change significantly after eating.

Insulin-like Growth Factor 1, also known as IGF-1, is used as a biomarker for blood levels of human growth hormone. It is a hormone produced by the liver and other tissues which affects growth-promoting activities and glucose metabolism. The blood level of IGF-1 appears to fluctuate over 24 hours and is found to be related to growth hormone secretion.